Core Rsch Facil Technician III - NextGen DNA Sequencing

Core Rsch Facil Technician III

Technical Functions

• Performs QC of samples submitted to the NGS lab. This includes assessing the quantity and quality of materials to determine suitability for NextGen DNA sequencing procedures.

• Submits samples for qPCR to the Gene Expression core

• Constructs Illumina libraries for sequencing on Illumina platforms

• Properly tracks samples and documents laboratory work

• Communicates with supervisor and other core personnel about experimental results in a timely and professional manner

• Works with the supervisor to define deliverables and timelines for project completion

• Tests, troubleshoots, and implements new protocols for library construction, DNA shearing, sample cleanup, and size selection, using novel procedures as they become available in the literature or from vendors

• Contributes to the preparation, updating, and organization of Standard Operating Procedures (SOPs)

General Lab Operation and Maintenance Functions

• Performs maintenance tasks associated with the upkeep of the NGS lab, such as the proper disposal of biohazard trash, receiving and storing of samples and reagents

• Monitors reagents and consumables and regularly updates inventories

• Assists with the administrative duties necessary for proper handling of receipts and orders

• Revision of sample sheets, tracking, and disposal of samples in MyBiotech

• Freeze and fridge maintenance and documentation in MyBiotech

• Other duties as assigned

$45,000 - $46,000

BA/BS in a related scientific field, or an equivalent combination of education and experience.
Core or technology-specific licensure and/or certifications, where applicable. Additionally, yearly certificate and compliance training, if required.

The preferred applicant will have a BS/BA in Molecular Biology or a related scientific field with familiarity of in basic techniques and instrumentation used in the molecular biology laboratory, including specific NGS procedures that include (but are not limited to):

• Genomic DNA purification, quantification, and manipulation; PCR; NGS DNA library construction and quality assessment/evaluation.
• Use of robotics for high-throughput processing of nucleic acids and DNA library samples.
• Hands-on experience with Illumina, PacBio, and/or ONT NGS instrumentation and technology.
• Basic laboratory citizenship, including organization of equipment and supply inventory.
• Instrument run coordination and maintenance.
• Preparation of common buffers and solutions.
• Computer literacy in Windows/UNIX systems, Microsoft Office package.
• Familiarity with utilizing Standard Operating Procedures (SOPs), iLAB, and laboratory information management system (LIMS).

In order to be considered, you must upload your cover letter and resume.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.